Useful Links & Tools
- MSDS
- Specification Sheet
- Certificate of Analysis
- Certificate of Origin
- B3795 - Datasheet (83 KB)
- Cytokine Receptors Tumor Necrosis Receptor Family (203 KB )
- Bulk Quote
- Similar Products
B3795
Sigma
Brain-derived neurotrophic factor human
lyophilized powder, recombinant, expressed in Escherichia coli, cell culture tested
| Synonym: | BDNF |
| MDL number: | MFCD00285579 |
Description
| Analysis Note | The bioactivity is determined by its ability to stimulate the proliferation of rat C6 cells. |
| Biochem/physiol Actions | Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells. |
| Physical form | The product is lyophilized from a sterile 0.2 micron filtered solution containing 0.1% TFA with 250 μg BSA |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Barde, Y.A., Nicola, N., The nerve growth factor family−neurotrophins. Guidebook to Cytokines and Their Receptors New York, N.Y, , (1994), 140-143 |
| Callard, R., and Gearing, A. The Cytokine Facts Book New York, NY , (1994) |
